Journal: Cancer Research

Article Title: Gene Therapy with Secretory Leukoprotease Inhibitor Promoter-Controlled Replication-Competent Adenovirus for Non-Small Cell Lung Cancer

doi: 10.1158/0008-5472.can-03-2549

Figure Lengend Snippet: Fig. 1. Secretion of secretory leukoprotease inhibitor (SLPI) from various human cell lines, construction of AdSLPI.E1AdB, and E1A protein expression and replication of adenovirus in cancer cells infected with AdSLPI.E1AdB in vitro. A, SLPI secretion from human cell lines. The level of SLPI protein in the medium from nine human cell lines [NHBE (normal human bronchial epithelium), A549 (lung adenocarcinoma), H358 (lung adenocarcinoma), HS24 (lung squamous cell carcinoma), SBC-3 (small cell lung cancer), HepG2 (hepatocellular carcinoma), HT1080 (sarcoma), SAOS-2 (osteosarcoma), and INR-90 (normal fibroblast)] was determined by using an ELISA kit for human SLPI protein. B, a construct of AdSLPI.E1AdB. E1A region controlled by the SLPI promoter and E1B-19K driven by the CMV promoter composed the expression cassette of the Ad5 vector. C, Western blotting of E1A protein. Cell lysates were subjected to Western blot analysis using anti-adenovirus E1A protein. D, Southern blotting of the E4 region. DNA extracted from A549 or HepG2 cells was analyzed by Southern blotting using E4 probe. C and D, A549 or HepG2 cells were infected with either AdSLPI.LacZ (Lanes 1), AdSLPI.E1AdB (Lanes 2), or AxE1AdB (Lanes 3).

Article Snippet: To evaluate the secretion of SLPI protein from the various cell lines, concentrations of SLPI protein in the culture medium after 72 h of incubation (1 106 cells/10-cm dish) were determined by an ELISA kit for human SLPI (Quantikine; R&D Systems, Inc.).

Techniques: Expressing, Infection, In Vitro, Enzyme-linked Immunosorbent Assay, Construct, Plasmid Preparation, Western Blot, Southern Blot

Journal: BMC Pediatrics

Article Title: Correlation of biochemical markers and inflammatory cytokines in autism spectrum disorder (ASD)

doi: 10.1186/s12887-024-05182-3

Figure Lengend Snippet: The minimum and maximum values, the median, and the IQR for each marker. The IQR represents the middle 50% of the data, with the 25th percentile and the 75th percentile values

Article Snippet: Human ELISA Kits were used to measure CRP (Quantikine, R&D Systems, cat. DCRP00B, USA, Sensitivity: 0.022 ng/mL), TNF-α (Immunological Sciences, cat. IK4185, Sensitivity: 1 pg/ml), TGF-β1(Diaclone Human TGF-β1 ELISA kit, Sensitivity: 9 pg/mL), IL-1β (Quantikine, R&D Systems, cat. HSLB00D, USA, Sensitivity: 0.063 pg/mL), IL-10 (Quantikine, R&D Systems, cat. HS100C, USA, Sensitivity: 0.17 pg/m), Il-8 (MabTech Human IL-8 ELISA kit, Sensitivity: 2 pg/mL), and IL-6 (Quantikine, R&D Systems, cat.HS600C, USA, sensitivity: 0.09 pg/mL) in the serum samples of ASD patients and control group, according to the Kit manufacturer.

Techniques: Marker

Journal: BMC Pediatrics

Article Title: Correlation of biochemical markers and inflammatory cytokines in autism spectrum disorder (ASD)

doi: 10.1186/s12887-024-05182-3

Figure Lengend Snippet: Comparing the serum concentrations of CRP, TNF-α, TGF-β, IL-1β, and IL-6 between ASD patients and control individuals

Article Snippet: Human ELISA Kits were used to measure CRP (Quantikine, R&D Systems, cat. DCRP00B, USA, Sensitivity: 0.022 ng/mL), TNF-α (Immunological Sciences, cat. IK4185, Sensitivity: 1 pg/ml), TGF-β1(Diaclone Human TGF-β1 ELISA kit, Sensitivity: 9 pg/mL), IL-1β (Quantikine, R&D Systems, cat. HSLB00D, USA, Sensitivity: 0.063 pg/mL), IL-10 (Quantikine, R&D Systems, cat. HS100C, USA, Sensitivity: 0.17 pg/m), Il-8 (MabTech Human IL-8 ELISA kit, Sensitivity: 2 pg/mL), and IL-6 (Quantikine, R&D Systems, cat.HS600C, USA, sensitivity: 0.09 pg/mL) in the serum samples of ASD patients and control group, according to the Kit manufacturer.

Techniques: Control

Journal: BMC Pediatrics

Article Title: Correlation of biochemical markers and inflammatory cytokines in autism spectrum disorder (ASD)

doi: 10.1186/s12887-024-05182-3

Figure Lengend Snippet: Correlation matrix of inflammatory markers in the ASD patients

Article Snippet: Human ELISA Kits were used to measure CRP (Quantikine, R&D Systems, cat. DCRP00B, USA, Sensitivity: 0.022 ng/mL), TNF-α (Immunological Sciences, cat. IK4185, Sensitivity: 1 pg/ml), TGF-β1(Diaclone Human TGF-β1 ELISA kit, Sensitivity: 9 pg/mL), IL-1β (Quantikine, R&D Systems, cat. HSLB00D, USA, Sensitivity: 0.063 pg/mL), IL-10 (Quantikine, R&D Systems, cat. HS100C, USA, Sensitivity: 0.17 pg/m), Il-8 (MabTech Human IL-8 ELISA kit, Sensitivity: 2 pg/mL), and IL-6 (Quantikine, R&D Systems, cat.HS600C, USA, sensitivity: 0.09 pg/mL) in the serum samples of ASD patients and control group, according to the Kit manufacturer.

Techniques:

Journal: BMC Pediatrics

Article Title: Correlation of biochemical markers and inflammatory cytokines in autism spectrum disorder (ASD)

doi: 10.1186/s12887-024-05182-3

Figure Lengend Snippet: Correlation matrix of inflammatory markers in the control group

Article Snippet: Human ELISA Kits were used to measure CRP (Quantikine, R&D Systems, cat. DCRP00B, USA, Sensitivity: 0.022 ng/mL), TNF-α (Immunological Sciences, cat. IK4185, Sensitivity: 1 pg/ml), TGF-β1(Diaclone Human TGF-β1 ELISA kit, Sensitivity: 9 pg/mL), IL-1β (Quantikine, R&D Systems, cat. HSLB00D, USA, Sensitivity: 0.063 pg/mL), IL-10 (Quantikine, R&D Systems, cat. HS100C, USA, Sensitivity: 0.17 pg/m), Il-8 (MabTech Human IL-8 ELISA kit, Sensitivity: 2 pg/mL), and IL-6 (Quantikine, R&D Systems, cat.HS600C, USA, sensitivity: 0.09 pg/mL) in the serum samples of ASD patients and control group, according to the Kit manufacturer.

Techniques: Control

Journal: The Prostate

Article Title: PKC? activation mediates angiogenesis via NADPH oxidase activity in PC-3 prostate cancer cells

doi: 10.1002/pros.21310

Figure Lengend Snippet: (A) The level of angiogenesis at the end of the 5-week growth was measured with immunofluorescence by staining blood vessels with anti-CD31 monoclonal antibodies conjugated with FITC (n=3 each, *; p<0.05, Scale bar: 10μm). (B) One week after PC-3 cell injection, mice were implanted with osmotic pumps with control peptide (TAT) or ψδRACK at 38 mg/kg/day (30mM) for 4 weeks. HIF-1α levels were measured by Western blot analyses using whole cell lysates from tumors (Figure 2B left, *; p<0.05 t test, n=3 each). GAPDH was used as a loading control. (C) Next, human VEGF concentration was measured in the serum from mice treated with control peptide (TAT) or ψδRACK by ELISA (Figure 2C left, *; p<0.05, n=4 each). Mouse VEGF concentration was measured in the serum from mice treated with control peptide (TAT) or ψδRACK by ELISA (Figure 2C, right).

Article Snippet: The concentration of VEGF in the serum was measured using an ELISA kit (human and mouse VEGF immunoassay, Quantikine, R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions.

Techniques: Immunofluorescence, Staining, Bioprocessing, Injection, Control, Western Blot, Concentration Assay, Enzyme-linked Immunosorbent Assay